DNA sequencing - 2025 ACF Fall

Tossup

One form of this technique uses tagmentation to fragment samples and add adapters simultaneously (10[1])for library preparation. (10[2])Solexa pioneered a form of this technique in which a sample in a flow cell (10[1])is subjected to bridge amplification. (-5[2])Samples (10[1]-5[1])are treated with (-5[1])bisulfite in one form (10[2])of this (-5[1])technique (10[2])to determine methylation patterns. (10[1])Another (-5[1])form of this technique (10[1]-5[1])uses capillary (10[1])electrophoresis (-5[1])to migrate samples (-5[2])through a fluorescence (-5[4])detector (10[1]-5[3])to generate (10[1]-5[1])a chromatogram. (10[2]-5[3])That (-5[1])form (10[1]-5[1])of this technique (-5[1])relies (10[1])on chain (10[1]-5[1])termination (-5[3])by randomly (10[1]-5[1])incorporated (10[2]-5[3])ddNTPs (10[4]-5[3])and is named for (-5[1])Sanger. (10[1])For (10[1])10 (10[1])points, (10[5]-5[1])name (10[1])this technique (10[4])used (10[1])to determine the order (10[1])of nucleotides (10[3]-5[2])in (10[1])DNA. (10[2])■END■ (10[40]0[26])

ANSWER: DNA sequencing [accept genome sequencing; accept Sanger sequencing; accept bisulfite sequencing; accept next-generation sequencing or NGS or massive parallel sequencing or massively parallel sequencing or second-generation sequencing; accept DNA sequencing library or DNA sequencing libraries; accept ATAC-seq or Assay for Transposase-Accessible Chromatin using sequencing; accept Illumina dye sequencing; accept Solexa sequencing; reject “mapping” or “genome mapping”; reject “RNA sequencing” or “peptide sequencing”]

= Average correct buzz position

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Buzzes

Player	Team	Opponent	Position	Value
Sammy Carstens	Rochester	RIT B	13	10
Ilyas Choukri	Virginia Tech A	William & Mary A	16	10
Eric Yang	Purdue A	WashU Grizzlers	16	10
Jeremy Kim	Toronto F	McMaster A	31	10
Aaryan Sumesh	Berkeley Archer Queen	Berkeley Skeleton King	36	-5
Daniel Chen	Emory A	Tennessee Tech	36	-5
Charlie Ruff	McGill A	Ottawa B	37	10
Jacob Goodson	Ohio State A	Ohio State D	37	-5
Niyanthi Yamunan	Georgia Tech E	Tennessee B	40	-5
Advai Srinivasan	UC Irvine	UCLA C	44	10
Kelvin Ni	NYU A	George Mason C	44	10
Jesse Mindel	NCSU A	Virginia A	46	-5
March Delfin	UCSD A	Claremont A	47	10
Claire Wilson	Lehigh B	Vassar B	47	10
Elizabeth Ziberman	Stony Brook	NYU B	51	10
Alma Gazzaniga	UNC B	Duke A	52	-5
Ethen Sun	Toronto C	Toronto E	56	10
Sia Kalia	Warwick	Imperial C	56	-5
Laura Bullock	Sheffield A	Edinburgh D	58	10
Sarah Silverman	Claremont C	Claremont B	59	-5
Emily Kadolph	Virginia Tech B	UNC A	62	-5
Omer Korkmaz	Johns Hopkins	Rutgers D	62	-5
Nikola Wu	UCLA D	UCLA F	65	-5
Wyatt Johnson	CWRU B	Ohio State B	65	-5
Joseph Strom	Louisville B	Michigan State D	65	-5
Fern Fang	VCU A	UNC C	65	-5
Andrew Nucci	UCLA B	UCLA E	66	10
Bonham Buchannon	Louisville D	CWRU A	66	-5
Arjun Saulnier	CWRU C	Michigan State C	66	-5
Austin Tran	Rose-Hulman A	Purdue B	66	-5
Darren Kellis	Vanderbilt A	Mississippi State A	68	10
Isabel Dent	Sewanne	Auburn B	68	-5
James Xiao	Toronto A	Waterloo B	70	-5
Anna Liao	Michigan B	WVU A	70	10
Caleb Slusher	South Carolina A	Auburn C	70	10
Kamil Boehm	Cambridge A	Cambridge E	70	-5
Nayaaz Hashim	Oxford	Southampton	70	-5
Eli O'Sick Johnson	Binghamton C	RIT A	71	-5
Susanna Liao	Waterloo A	Toronto G	72	10
Adithya Parat	McMaster C	Toronto H	72	-5
Chris Chang	USC A	USC B	75	-5
Sarah Dong	Michigan A	Michigan C	76	10
Ben Stras	Wisconsin C	Minnesota	78	10
Anne Fjeld	Alberta A	Washington Chinook	78	-5
Joe Paterson	Michigan State A	Michigan D	79	-5
Nitish Sudhakar	Georgia Tech A	Georgia Tech D	79	-5
Musa Ahmed	Edinburgh C	Edinburgh A	79	-5
Pratham Bhanushali	WVU B	CWRU D	81	10
Misty Chen	Purdue C	Chicago A	81	-5
Xavier Bornhorst	Maryland A	George Mason B	82	10
Johnathan Ahdout	Princeton	Rutgers C	82	-5
Jonathan Ho	Alberta B	British Columbia C	82	10
Jeremy Scurlock	Chipola College	Montevallo	82	-5
Yasmin Campbell	Cambridge B	LSE	82	-5
Carly Hemani	JMU A	Tusculum A	83	-5
Daniel Abrams	Rutgers B	Rutgers A	83	-5
Francis Dinh	Illinois Canada	Illinois Champaign	83	-5
Jiping Fang	Illinois Blue	Indiana A	83	10
Zach Majorowicz	St. Thomas	Wisconsin B	83	10
Wesley Stine	Clemson A	Georgia Tech B	83	10
Juliette Flatau	Imperial A	Bristol	83	10
Brayden Zhang	Vanderbilt B	Tennessee A	87	-5
Liam Rojas	Virginia A	NCSU A	88	10
Alden Thompson	Carleton A	McGill E	89	10
Tristan Scarlett	Ottawa D	McGill C	90	10
Stephen Ottewell	Toronto B	McMaster B	91	-5
Snehan Saroch	Louisville A	Ohio State C	91	10
Rohit Chintala	Maryland B	NYU C	91	10
Sebastian Llaca	Wisconsin A	Macalester	91	10
Daniel Lu	Washington Pink	Washington Chum	91	10
Judah Solomon	Imperial D	Cambridge C	91	10
Sophia Jagels	William & Mary B	UNC D	92	10
Galen Mueller	McGill B	Ottawa A	94	10
Lucas Hall	Waterloo C	Brock A	94	10
Will Marcotte	Toronto D	Guelph A	94	10
Simon Choist	Queen's A	Toronto I	94	10
Dustin Sanders	Chicago B	Indiana B	95	10
Ritisha Dey	Stanford A	Berkeley Little Prince	99	10
Yechan Park	Berkeley Boss Bandit	Stanford B	101	10
Ethan Ririe	UCSD B	UC Santa Barbara	101	10
Cooper Morris	Columbia B	Columbia A	101	-5
Matthew Bucek	George Mason A	Maryland C	101	-5
Aaron Wang	Cornell A	Cornell B	101	10
Andrew Boardman	Cambridge D	Cardiff	102	10
Varun Ramanathan	Michigan D	Michigan State A	103	10
Camden Walker	WashU Grolars	Iowa B	103	10
Suren Grigorian	Berkeley Mighty Miner	UCSC	104	0
Huy Lai	UCSC	Berkeley Mighty Miner	104	0
Hansen Jin	Berkeley Skeleton King	Berkeley Archer Queen	104	10
Jackson Crickard	Claremont B	Claremont C	104	10
Rohan Soni	UCLA F	UCLA D	104	0
Taman Truong	USC B	USC A	104	0
Gus Morin-Strom	Carleton B	Ottawa C	104	0
Sam Montgomery	Ottawa C	Carleton B	104	0
Gavin Ge	Waterloo B	Toronto A	104	10
Addison Wiggins	South Florida A	Florida A	104	10
Matthew Andersen	Florida B	UCF A	104	0
Heidi Moyer	ERAU Daytona A	Florida Tech A	104	10
Miles Phillips	Florida Tech B	Florida State A	104	0
Pranet Sirikonda	Ohio State B	CWRU B	104	10
Nico Pascual	Michigan State B	Kentucky	104	0
Nishad Joshi	Kentucky	Michigan State B	104	10
Enina Edosomwan	CWRU A	Louisville D	104	10
Maya Hunt	Michigan State C	CWRU C	104	0
Andrea Owusu	Duke A	UNC B	104	10
Caleigh Greene	Tusculum A	JMU A	104	10
Brendan Karg	JMU B	William & Mary C	104	10
Nick Smemoe	UNC A	Virginia Tech B	104	10
Vedang Singhal	UNC C	VCU A	104	10
Kevin Cronk	Virginia Tech C	Liberty C	104	10
Andrew Wu	Columbia A	Columbia B	104	0
John Shelly	Rowan	Lehigh A	104	10
Aarav Vasanthan	Rutgers A	Rutgers B	104	10
Aaron Henry	Maryland C	George Mason A	104	10
Hitansh Malaviya	Rutgers C	Princeton	104	10
Alex Wong	Rutgers D	Johns Hopkins	104	0
Vedh Ramesh	Penn	Vassar A	104	10
Saswat Sasha Tripathi	Chicago A	Purdue C	104	10
David Black	Illinois Champaign	Illinois Canada	104	0
Aarnav Vyas	Illinois Orange	Illinois Urbana	104	0
Samarth Majmudar	Illinois Urbana	Illinois Orange	104	0
Beckett Pursey	Purdue B	Rose-Hulman A	104	10
Alex Dzurick	William Woods University A	Purdue D	104	10
Alex Matchinski	Michigan Tech	Iowa State	104	10
Christopher Bradley	Southern Connecticut State University A	Boston College B	104	10
Brandon Wiltse	Washington Chinook	Alberta A	104	10
Rae Meyers	Arizona State A	Boise State	104	10
Luke Houtz	Arizona State B	British Columbia B	104	0
Henry Rutherford-Braun	British Columbia B	Arizona State B	104	10
Gavin Morrow	Washington Steelhead	British Columbia A	104	0
Brendan Liaw	British Columbia A	Washington Steelhead	104	10
Vishnu Sharma	Texas	Washington Coho	104	0
Deeksha Easwar	Washington Sockeye	Washington Masu	104	0
Haylee Weisner	Washington Masu	Washington Sockeye	104	10
Ethan Miniard	Montevallo	Chipola College	104	0
Moses Dong	Georgia Tech F	Clemson B	104	10
Jacie Bradley	Clemson C	Alabama A	104	10
Martin Brandenburg	Georgia A	Auburn A	104	10
Sonali Srikanth	Georgia Tech D	Georgia Tech A	104	0
Richard D’Angelo	Tennessee B	Georgia Tech E	104	0
Daniel Slater	Tennessee Tech	Emory A	104	0
Andreas Ktori	Durham A	York	104	0
Benjamin Turner	York	Durham A	104	10
Jacob Coyne	Durham C	Durham D	104	10
James Headon	Edinburgh A	Edinburgh C	104	10
Stephen Donoghue	Sheffield B	Durham B	104	10
Rory Moloney	Cambridge E	Cambridge A	104	0
Vishrut Pisupati	Imperial B	Birmingham	104	10
James Carrick-Lawson	Southampton	Oxford	104	10
Judah Garlic	Cornell C	Binghamton A	104	10
Eric Zhu	Cornell D	ESF	104	10
Will Hoover	RIT A	Binghamton C	104	0

Summary

Tournament	Edition	TUH	Conv. %	Neg %	Average Buzz
UK (North)	UK	5	100%	20%	94.80
UK (South)	UK	8	63%	50%	96.80
Northern California	US	4	75%	25%	101.33
Southern California	US	7	71%	43%	72.40
Eastern Canada (1)	US	5	80%	0%	77.50
Eastern Canada (2)	US	9	78%	33%	77.86
Florida	US	4	50%	0%	104.00
Great Lakes	US	11	73%	55%	91.63
Lower Mid-Atlantic	US	9	100%	56%	91.11
Upper Mid-Atlantic	US	1	0%	100%
Upper Mid-Atlantic	US	9	89%	33%	78.38
Upper Mid-Atlantic	US	2	100%	50%	104.00
Midwest	US	9	78%	33%	87.00
North	US	4	100%	0%	89.00
Northeast	US	1	100%	0%	104.00
Pacific	US	8	88%	13%	99.00
Southeast	US	12	50%	50%	88.83
Upstate NY	US	5	80%	20%	80.50