Round 5: Tossup 17
One form of this technique uses tagmentation to fragment samples and add adapters simultaneously for library preparation. Solexa pioneered a form of this technique in which a sample in a flow cell is subjected to bridge amplification. Samples are treated with bisulfite in one form of this technique to determine methylation patterns. Another form of this technique uses capillary electrophoresis to migrate samples through a fluorescence detector to generate a chromatogram. That form of this technique relies on chain termination by randomly incorporated ddNTPs and is named for Sanger. For 10 points, name this technique used to determine the order of nucleotides in DNA. ■END■
ANSWER: DNA sequencing [accept genome sequencing; accept Sanger sequencing; accept bisulfite sequencing; accept next-generation sequencing or NGS or massive parallel sequencing or massively parallel sequencing or second-generation sequencing; accept DNA sequencing library or DNA sequencing libraries; accept ATAC-seq or Assay for Transposase-Accessible Chromatin using sequencing; accept Illumina dye sequencing; accept Solexa sequencing; reject “mapping” or “genome mapping”; reject “RNA sequencing” or “peptide sequencing”]
<Nebraska B, Biology> | Packet E
= Average correct buzzpoint
Buzzes
| Player | Team | Opponent | Buzzpoint | Value |
|---|---|---|---|---|
| Laura Bullock | Sheffield A | Edinburgh D | 58 | 10 |
| Musa Ahmed | Edinburgh C | Edinburgh A | 79 | -5 |
| Andreas Ktori | Durham A | York | 104 | 0 |
| Benjamin Turner | York | Durham A | 104 | 10 |
| Jacob Coyne | Durham C | Durham D | 104 | 10 |
| James Headon | Edinburgh A | Edinburgh C | 104 | 10 |
| Stephen Donoghue | Sheffield B | Durham B | 104 | 10 |
Summary
| Tournament | Edition | Match | Heard | Conv. % | Neg % | Avg. Buzz |
|---|---|---|---|---|---|---|
| Northern California | Main | ✓ | 4 | 75% | 25% | 101.33 |
| Southern California | Main | ✓ | 7 | 71% | 43% | 72.40 |
| Eastern Canada (1) | Main | ✓ | 5 | 80% | 0% | 77.50 |
| Eastern Canada (2) | Main | ✓ | 9 | 78% | 33% | 77.86 |
| Florida | Main | ✓ | 4 | 50% | 0% | 104.00 |
| Great Lakes | Main | ✓ | 11 | 73% | 55% | 91.63 |
| Lower Mid-Atlantic | Main | ✓ | 9 | 100% | 56% | 91.11 |
| Upper Mid-Atlantic | Main | ✓ | 1 | 0% | 100% | |
| Upper Mid-Atlantic | Main | ✓ | 9 | 89% | 33% | 78.38 |
| Upper Mid-Atlantic | Main | ✓ | 2 | 100% | 50% | 104.00 |
| Midwest | Main | ✓ | 9 | 78% | 33% | 87.00 |
| North | Main | ✓ | 4 | 100% | 0% | 89.00 |
| Northeast | Main | ✓ | 1 | 100% | 0% | 104.00 |
| Pacific | Main | ✓ | 8 | 88% | 13% | 99.00 |
| South Central | Main | ✓ | 5 | 100% | 60% | 94.20 |
| Southeast | Main | ✓ | 12 | 50% | 50% | 88.83 |
| Upstate NY | Main | ✓ | 5 | 80% | 20% | 80.50 |
| UK (North) | UK | ✓ | 5 | 100% | 20% | 94.80 |
| UK (South) | UK | ✓ | 8 | 63% | 50% | 96.80 |