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One form of this technique uses tagmentation to fragment samples and add adapters simultaneously for library preparation. Solexa pioneered a form of this technique in which a sample in a flow cell is subjected to bridge amplification. Samples (-5[1])are treated with bisulfite in one form of this technique to determine methylation patterns. Another form of this technique uses capillary electrophoresis to migrate samples through a fluorescence (-5[2])detector (-5[2])to generate a chromatogram. (10[1])That form of this technique relies (10[1])on chain termination (-5[1])by randomly (10[1])incorporated ddNTPs and is named for Sanger. For 10 points, (10[1])name this technique used to determine the order of nucleotides in DNA. (10[1])■END■ (10[3]0[2])

ANSWER: DNA sequencing [accept genome sequencing; accept Sanger sequencing; accept bisulfite sequencing; accept next-generation sequencing or NGS or massive parallel sequencing or massively parallel sequencing or second-generation sequencing; accept DNA sequencing library or DNA sequencing libraries; accept ATAC-seq or Assay for Transposase-Accessible Chromatin using sequencing; accept Illumina dye sequencing; accept Solexa sequencing; reject “mapping” or “genome mapping”; reject “RNA sequencing” or “peptide sequencing”]
<Nebraska B, Biology>
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Buzzes

PlayerTeamOpponentPositionValue
Jacob GoodsonOhio State AOhio State D37-5
Wyatt JohnsonCWRU BOhio State B65-5
Joseph StromLouisville BMichigan State D65-5
Bonham BuchannonLouisville DCWRU A66-5
Arjun SaulnierCWRU CMichigan State C66-5
Anna LiaoMichigan BWVU A7010
Sarah DongMichigan AMichigan C7610
Joe PatersonMichigan State AMichigan D79-5
Pratham BhanushaliWVU BCWRU D8110
Snehan SarochLouisville AOhio State C9110
Varun RamanathanMichigan DMichigan State A10310
Pranet SirikondaOhio State BCWRU B10410
Nico PascualMichigan State BKentucky1040
Nishad JoshiKentuckyMichigan State B10410
Enina EdosomwanCWRU ALouisville D10410
Maya HuntMichigan State CCWRU C1040

Summary

TournamentEditionTUHConv. %Neg %Average Buzz
UK (North)UK5100%20%94.80
UK (South)UK863%50%96.80
Northern CaliforniaUS475%25%101.33
Southern CaliforniaUS771%43%72.40
Eastern Canada (1)US580%0%77.50
Eastern Canada (2)US978%33%77.86
FloridaUS450%0%104.00
Great LakesUS1173%55%91.63
Lower Mid-AtlanticUS9100%56%91.11
Upper Mid-AtlanticUS10%100%
Upper Mid-AtlanticUS989%33%78.38
Upper Mid-AtlanticUS2100%50%104.00
MidwestUS978%33%87.00
NorthUS4100%0%89.00
NortheastUS1100%0%104.00
PacificUS888%13%99.00
SoutheastUS1250%50%88.83
Upstate NYUS580%20%80.50