Bonus
Bacteria transformed with the pGLO (“p-glow”) plasmid can express this protein only when arabinose is present. For 10 points each:
[10h] Name this protein, whose discovery won Tsien, Shimomura, and Chalfie the 2008 Nobel Prize in Chemistry. This protein, which was first isolated from Aequorea victoria, has an emission peak at roughly 509 nanometers.
ANSWER: GFP [or green fluorescent protein]
[10m] The pGLO plasmid’s bla gene codes for an enzyme that hydrolyzes the beta-lactam type of these compounds. Pen-Strep is a cocktail of these compounds widely used in cell culture to prevent contamination.
ANSWER: antibiotics [accept antimicrobials; prompt on penicillin or streptomycin or penicillin-streptomycin by asking “what more general type of compound is that?”]
[10e] Like most circular plasmids, the pGLO plasmid initiates this process at its ori (“OR-ee”) site. This semiconservative process uses polymerases to duplicate strands of DNA.
ANSWER: DNA replication [accept origin of replication]
<Editors, Biology>
| Editions | Heard | PPB | Easy % | Medium % | Hard % |
|---|---|---|---|---|---|
| 2 | 105 | 14.57 | 70% | 53% | 23% |
Conversion
| Team | Opponent | Part 1 | Part 2 | Part 3 | Total | Parts |
|---|---|---|---|---|---|---|
| Binghamton A | RIT B | 0 | 0 | 0 | 0 | |
| Cornell A | Cornell D | 0 | 10 | 10 | 20 | ME |
| Cornell C | Binghamton B | 0 | 0 | 10 | 10 | E |
| RIT A | ESF | 0 | 0 | 0 | 0 |
Summary
| Tournament | Edition | Exact Match? | Heard | PPB | Easy % | Medium % | Hard % |
|---|---|---|---|---|---|---|---|
| UK (North) | UK | Y | 4 | 17.50 | 75% | 75% | 25% |
| UK (South) | UK | Y | 5 | 6.00 | 20% | 40% | 0% |
| Northern California | US | Y | 4 | 20.00 | 75% | 100% | 25% |
| Southern California | US | Y | 6 | 15.00 | 83% | 50% | 17% |
| Eastern Canada (1) | US | Y | 4 | 15.00 | 100% | 50% | 0% |
| Eastern Canada (2) | US | Y | 7 | 15.71 | 57% | 57% | 43% |
| Florida | US | Y | 1 | 10.00 | 100% | 0% | 0% |
| Great Lakes | US | Y | 11 | 18.18 | 73% | 64% | 46% |
| Lower Mid-Atlantic | US | Y | 7 | 18.57 | 100% | 57% | 29% |
| Upper Mid-Atlantic | US | Y | 9 | 13.33 | 89% | 33% | 11% |
| Upper Mid-Atlantic | US | Y | 1 | 10.00 | 0% | 0% | 100% |
| Midwest | US | Y | 9 | 13.33 | 44% | 56% | 33% |
| North | US | Y | 4 | 10.00 | 50% | 50% | 0% |
| Northeast | US | Y | 11 | 18.18 | 91% | 73% | 18% |
| Pacific | US | Y | 8 | 12.50 | 63% | 38% | 25% |
| Southeast | US | Y | 10 | 13.00 | 60% | 50% | 20% |
| Upstate NY | US | Y | 4 | 7.50 | 50% | 25% | 0% |